70-620
MilliporeSigma™ NFκB p65 EZ-TFA Transcription Factor Assay
Manufacturer: MilliporeSigma™
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Content And Storage
Store kit components at the temperatures indicated on the labels and insert until their expiration dateStore HeLa Whole Cell Extract at -80°C. Avoid repeated freeze-thaw cycles. Stable at -80°C for 6 months
Detection Method
Chemiluminescence
For Use With (Application)
Affinity Binding Assay, ELISA, Activity Assay
Quantity
1 Plate
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Description
- Provided in a 96-well format When the Capture Probe, a double stranded biotinylated oligonucleotide is incubated together, the active form of NFkB contained in the nuclear extract binds to its consensus sequence After incubation, the extract/probe/buffer mixture is then directly transferred to the streptavidin coated plate The biotinylated double stranded oligonucleotide bound by active NFkB protein is immobilized and any inactive, unbound material is washed away The bound NFkB transcription factor subunits, p50 and p65, are detected with specific primary antibodies, a Rabbit anti-NFkB p50 (active form) and a Rabbit anti-NFkB p65 An HRP-conjugated secondary antibody is then used for detection and provides sensitive colorimetric detection that can be read in a spectrophotometric plate reader
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Content And Storage: Store kit components at the temperatures indicated on the labels and insert until their expiration dateStore HeLa Whole Cell Extract at -80°C. Avoid repeated freeze-thaw cycles. Stable at -80°C for 6 months
Detection Method: Chemiluminescence
For Use With (Application): Affinity Binding Assay, ELISA, Activity Assay
Quantity: 1 Plate
Content And Storage:
Store kit components at the temperatures indicated on the labels and insert until their expiration dateStore HeLa Whole Cell Extract at -80°C. Avoid repeated freeze-thaw cycles. Stable at -80°C for 6 months
Detection Method:
Chemiluminescence
For Use With (Application):
Affinity Binding Assay, ELISA, Activity Assay
Quantity:
1 Plate
Content And Storage: ≤-70°C
Detection Method: __
For Use With (Application): __
Quantity: 0.4 mL
Content And Storage:
≤-70°C
Detection Method:
__
For Use With (Application):
__
Quantity:
0.4 mL
Content And Storage: ≤-70°C
Detection Method: __
For Use With (Application): __
Quantity: 1 mL
Content And Storage:
≤-70°C
Detection Method:
__
For Use With (Application):
__
Quantity:
1 mL
Content And Storage: ≤-70°C
Detection Method: __
For Use With (Application): __
Quantity: 0.4 mL
Content And Storage:
≤-70°C
Detection Method:
__
For Use With (Application):
__
Quantity:
0.4 mL