7091871

Invitrogen™ eBioscience™ Calcium Sensor Dye eFluor™ 514

Membrane-permeable dye used to monitor changes in intracellular free calcium concentrations in the cell. Once eFluor™ 514 Calcium Sensor Dye enters the cell, cellular esterases cleave the AM group yielding a membrane-impermeable dye fluorescing at ≈520nm.

Manufacturer: Fischer Scientific

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Detection Method

Fluorescence

For Use With (Equipment)

Fluorescence Microscope, Flow Cytometer

Emission

490⁄514

For Use With (Application)

Calcium Assay

Product Type

Dye

Product Line

eBioscience™

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Description

  • Calcium binding affinity: Kd = 232 nM Molecular Weight: ≈1100 d Peak Excitation: 490nm Peak Emission: 514nm Reconstitute in high-quality, freshly opened DMSO Recommended concentration: 2 to 5 mM Once reconstituted, protect from light and store dessicated at −20°C; avoid freeze-thawing

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