Thermo Scientific™ PolarScreen™ ER Alpha Competitor Assay, Green

Description

• The PolarScreen™ ER (Estrogen Receptor) Alpha Competitor Assay Kit, Green, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential ER alpha ligands
• The kit uses insect cell-expressed, full-length, native (untagged) human estrogen receptor alpha (available separately, Cat
• No
• A15674) and a novel, high-affinity, fluorescent estrogen ligand (Fluormone ES2 Green) in a homogenous, mix-and-read assay format
• This kit produces a green color read-out; a red kit is also available
• Both kits suitably determine the IC50s of competitive compounds
• Color choice depends on the spectral properties of the compounds to be screened and the available instrumentation
• Complete & ready-to-use out of the box - all reagents included, just add your test compounds High throughput compatible - 800 x 20 μL fluorescence polarization assays in a 384-well plate Convenient - available in bulk sizes for larger screening needs Includes: 2 tubes full-length ER Alpha Recombinant Protein (store at -68 to -85°C) 1 tube of Fluormone ES2 Green Tracer (store at -5 to -30°C) 1 bottle of ES2 Screening Buffer (store at -5 to -30°C)