Invitrogen™ CellMask™ Orange Actin Tracking Stain

Description

• Stain and track intracellular F-actin with CellMask Orange Actin Tracking Stain in live or fixed cells in immunofluorescence (IF), immunocytochemistry (ICC), and immunohistochemistry (IHC) protocols, and in 3D cell culture
• The CellMask Orange Actin Tracking Stain fluorescently labels polymerized/filamentous actin (F-actin) in live or fixed cells because it is designed to readily permeate live cells, resulting in bright and uniform labeling
• CellMask Orange Actin Tracking Stain can be used to track F-actin in live cells for 24 hours or more
• Live cells stained with CellMask Orange Actin Tracking Stain can be fixed; the stain can also be used on already fixed cells
• Cell Mask Orange Actin Tracking Stain can be multiplexed with antibodies in IF, ICC, and IHC protocols, and can also be used in 3D cell culture for 3D imaging
• Features of Cell Mask Orange Actin Tracking Stain include the following: • Stains F-actin in live or fixed cells for fluorescence 2D monolayer or 3D imaging experiments • Can be multiplexed with antibodies in IF/ICC/IHC experiments • Fluorescence maxima (Excitation/Emission): 545/570 nm; can be detected with TRITC/RFP filter CellMask actin tracking stains are available in three colors: green (Cat
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• A57243, A57246), detected with a FITC/GFP filter (Ex 503 nm/Em 512 nm), orange (Cat
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• A57244, A57247) detected with a TRITC/RFP filter (Ex 545 nm/Em 570 nm), and deep red (Cat
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• A57245, A57248), detected with a CY5/Deep Red filter (Ex 652 nm/Em 669 nm)
• CellMask actin tracking stains detect F-actin in live or fixed cells or tissue with a targeting structure that closely resembles jasplakinolide
• They do not label globular actin (G-actin) or monomer actin (M-actin)
• CellMask actin tracking stains easily permeate live cell membranes and are retained within live cells after loading
• As demonstrated in the figures below, incubation with CellMask actin tracking stains has no detectable effect on viability of various cell lines after 24 hours of incubation
• In addition, CellMask actin tracking stains can be used to detect F-Actin in paraformaldehyde fixed cells or tissue (freshly fixed or cryo frozen only) and can be multiplexed with antibody detection.