A15893
LanthaScreen™ LanthaScreen™ TR-FRET ER beta Coactivator Assay Kit
Manufacturer: LanthaScreen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A15893-Each-of-1 | In Stock | ₹ 1,79,248.20 |
A15893 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,79,248.20
GST (18%): ₹ 32,264.676
Total Price: ₹ 2,11,512.876
Content And Storage
1 tube ER beta Ligand Binding Domain Recombinant Protein (store at -68 to -85°C)1 tube of Peptide (store at -5 to -30°C)2 bottles TR-FRET Buffer (store at -5 to -30°C)1 vial Tb-Anti-GST Ab (store at -5 to -30°C)1 tube of DTT (store at -5 to -30°C)
Product Line
LanthaScreen™
Type
Coactivator Assay Kit
For Use With (Application)
Co-Factor Interaction Assay, Nuclear Receptor Assay
Quantity
800 x 20 μL assays
Related Products
Description
- The LanthaScreen™ TR-FRET ER beta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential estrogen receptor (ER) beta ligands as agonists or antagonists of ligand-dependent coactivator recruitment
- The kit uses a ER beta ligand-binding domain (ER beta-LBD) that is tagged with glutathione-S-transferase (GST), a terbium (Tb)-labeled anti-GST antibody, and a fluorescein-labeled coactivator peptide in a homogenous mix-and-read assay format
- Agonist Mode - To run the LanthaScreen TR-FRET ER beta Coactivator Assay in agonist mode (to identify agonist compounds), ER beta-LBD is added to ligand test compounds followed by the addition of a mixture of fluorescein-coactivator peptide and Tb-anti-GST antibody
- After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound
- Based upon the biology of the ER beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide
- Antagonist Mode - When the LanthaScreen TR-FRET ER beta Coactivator Assay Kit is run in antagonist mode (to identify antagonist compounds), ER beta-LBD is added to ligand test compounds followed by the addition of a mixture of agonist, fluorescein-coactivator peptide, and Tb-anti-GST antibody
- The concentration of agonist used in this mode is the EC80 concentration as determined by first running the assay in agonist mode
- Includes: 1 tube ER beta Ligand Binding Domain Recombinant Protein (store at -68 to -85°C) 1 tube of Peptide (store at -5 to -30°C) 2 bottles TR-FRET Buffer (store at -5 to -30°C) 1 vial Tb-Anti-GST Ab (store at -5 to -30°C) 1 tube of DTT (store at -5 to -30°C)
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Content And Storage: 1 tube ER beta Ligand Binding Domain Recombinant Protein (store at -68 to -85°C)1 tube of Peptide (store at -5 to -30°C)2 bottles TR-FRET Buffer (store at -5 to -30°C)1 vial Tb-Anti-GST Ab (store at -5 to -30°C)1 tube of DTT (store at -5 to -30°C)
Product Line: LanthaScreen™
Type: Coactivator Assay Kit
For Use With (Application): Co-Factor Interaction Assay, Nuclear Receptor Assay
Quantity: 800 x 20 μL assays
Content And Storage:
1 tube ER beta Ligand Binding Domain Recombinant Protein (store at -68 to -85°C)1 tube of Peptide (store at -5 to -30°C)2 bottles TR-FRET Buffer (store at -5 to -30°C)1 vial Tb-Anti-GST Ab (store at -5 to -30°C)1 tube of DTT (store at -5 to -30°C)
Product Line:
LanthaScreen™
Type:
Coactivator Assay Kit
For Use With (Application):
Co-Factor Interaction Assay, Nuclear Receptor Assay
Quantity:
800 x 20 μL assays
Content And Storage: 1 tube ER beta Ligand Binding Domain Recombinant Protein (store at -68 to -85°C)1 tube of Fluormone ES2 (store at -5 to -30°C)2 bottles NR Buffer (store at -5 to -30°C)1 tube Tb-Anti-GST Ab (store at -5 to -30°C)1 tube of DTT (store at -5 to -30°C)
Product Line: LanthaScreen™
Type: Competitive Binding Kit
For Use With (Application): Competitive Binding Assay, Nuclear Receptor Assay
Quantity: 800 x 20 μL assays
Content And Storage:
1 tube ER beta Ligand Binding Domain Recombinant Protein (store at -68 to -85°C)1 tube of Fluormone ES2 (store at -5 to -30°C)2 bottles NR Buffer (store at -5 to -30°C)1 tube Tb-Anti-GST Ab (store at -5 to -30°C)1 tube of DTT (store at -5 to -30°C)
Product Line:
LanthaScreen™
Type:
Competitive Binding Kit
For Use With (Application):
Competitive Binding Assay, Nuclear Receptor Assay
Quantity:
800 x 20 μL assays
Content And Storage: 1 tube GR Ligand Binding Domain Recombinant Protein (store at -68 to -85°C)1 tube of Peptide (store at -5 to -30°C)2 bottles TR-FRET Buffer (store at -5 to -30°C)1 vial Tb-Anti-GST Ab (store at -5 to -30°C)1 tube of DTT (store at -5 to -30°C)
Product Line: LanthaScreen™
Type: Coactivator Assay Kit
For Use With (Application): Co-Factor Interaction Assay, Nuclear Receptor Assay
Quantity: 800 x 20 μL assays
Content And Storage:
1 tube GR Ligand Binding Domain Recombinant Protein (store at -68 to -85°C)1 tube of Peptide (store at -5 to -30°C)2 bottles TR-FRET Buffer (store at -5 to -30°C)1 vial Tb-Anti-GST Ab (store at -5 to -30°C)1 tube of DTT (store at -5 to -30°C)
Product Line:
LanthaScreen™
Type:
Coactivator Assay Kit
For Use With (Application):
Co-Factor Interaction Assay, Nuclear Receptor Assay
Quantity:
800 x 20 μL assays
Content And Storage: 1 tube PR Ligand Binding Domain Recombinant Protein (store at -68 to -85°C)1 tube of Peptide (store at -5 to -30°C)2 bottles TR-FRET Buffer (store at -5 to -30°C)1 tube Tb-Anti-GST Ab (store at -5 to -30°C)1 tube of DTT (store at -5 to -30°C)
Product Line: LanthaScreen™
Type: Coactivator Assay Kit
For Use With (Application): Co-Factor Interaction Assay, Nuclear Receptor Assay
Quantity: 800 x 20 μL assays
Content And Storage:
1 tube PR Ligand Binding Domain Recombinant Protein (store at -68 to -85°C)1 tube of Peptide (store at -5 to -30°C)2 bottles TR-FRET Buffer (store at -5 to -30°C)1 tube Tb-Anti-GST Ab (store at -5 to -30°C)1 tube of DTT (store at -5 to -30°C)
Product Line:
LanthaScreen™
Type:
Coactivator Assay Kit
For Use With (Application):
Co-Factor Interaction Assay, Nuclear Receptor Assay
Quantity:
800 x 20 μL assays