Molecular Probes™ Annexin V Conjugates for Apoptosis Detection

Description

• Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection
• Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry
• Annexin V has a high affinity for phosphatidylserine (PS), which becomes exposed on the outer leaflet of cells undergoing apoptosis
• Because of this affinity, fluorescently labeled annexin V reagents are commonly used in apoptosis research
• Annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, an indicator of intermediate stages of apoptosis
• The difference in fluorescence intensity between apoptotic and nonapoptotic cells stained with our fluorescent annexin V conjugates, as measured by flow cytometry, is typically about 100-fold
• In collaboration with Nexins Research BV, we provide the best and brightest annexin V conjugates available, including Alexa Fluor 350, 488, 555, 568, 594, 647, and 680 annexin V conjugates, as well as Annexin V APC, Biotin-X, FITC, Pacific Blue, and PE conjugates
• Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, one of the earliest indicators of apoptosis
• The Annexin V Pacific Blue conjugate is violet excitable, making it ideal for instruments with a violet laser and for multicolor experiments that include green- or red-fluorescent dyes
• The benefits of our annexin V conjugates include: • Conjugated to Invitrogen Alexa Fluor and eFluor dyes for brighter signals • Conjugates for all available lasers • Available as stand-alone reagents or easy-to-use kits Annexin V staining to detect apoptotic cells can only be done on live cells and tissue
• If samples are to be fixed post-staining, there are specific conditions required to achieve transient retention of signal
• These include use of an alcohol-free, aldehyde-based fixation method, use of buffers containing Ca2+ and avoidance of surfactants/detergents
• For your convenience, we also offer a concentrated annexin-binding buffer that facilitates the binding of annexin V to phosphatidylserine in apoptosis assays.