Molecular Probes™ CellROX™ Green Flow Cytometry Assay Kit

Description

• The CellROX Green Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells
• The kit includes the novel, fluorogenic CellROX Green Reagent, as well as SYTOX Red Dead Cell Stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS)
• The CellROX Green Flow Cytometry Assay Kit features: • Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS • Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowing easy multiplexing with other reagents • Simple protocol—cells can be stained in complete media or other appropriate buffer, no need for serum free media The CellROX Green Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state
• Upon oxidation, the reagent binds DNA and exhibits a strong fluorogenic signal that has absorption/emission maxima of 508/525 nm and remains localized to the nucleus and mitochondria
• When used together with the included SYTOX Red Dead Cell Stain, oxidatively stressed and nonstressed cells are reliably distinguished from dead cells by flow cytometry.