Invitrogen™ CellLight™ Lysosomes-GFP, BacMam 2.0

Description

• This ready-to-use construct is transduced/transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to Lamp1 (lysosomal associated membrane protein 1)
• You can observe lysosomes-GFP behavior in live cells using a fluorescent imaging system and standard FITC/GFP filter set
• Study staining of lysosomes, which does not depend upon organelle pH or some other parameter, that can be different from one cell type to other
• In addition, you can co-transduce/transfect more than one CellLight reagent and label live cells with multiple tracking or tracing dyes to image dynamic cellular processes
• Cells expressing CellLight constructs can also be fixed with formaldehyde for multiplexing with antibodies using immunocytochemical techniques
• BacMam Technology CellLight Lysosomes-GFP, BacMam 2.0, is a fusion construct of Lamp1 (lysosomal associated membrane protein 1) and emGFP, providing accurate and specific targeting to cellular lysosomes-GFP
• This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories
• BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity
• This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses
• Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting
• CellLight reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution
• Highly efficient: up to 90% transduction of wide range of mammalian cell lines, including primary cells, stem cells, and neurons Fast and convenient: simply add CellLight reagent to cells, incubate overnight, and image—or store frozen, assay-ready cells for later use Less toxic: CellLight reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose Flow Cytometer Laser Lines: 488 Cell Analysis, Cell Structure, Cell Viability, Proliferation & Function, Cellular Imaging, Chemotaxis & Cell Migration, Cytoskeleton, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunofluorescence Staining & Detection Order Info Shipping Condition: Wet Ice