Invitrogen™ Di-4-ANEPPS

Description

• Increases and decreases in membrane potential-referred to as membrane hyperpolarization and depolarization, respectively-play a central role in many physiological processes, including nerve-impulse propagation, muscle contraction, cell signaling and ion-channel gating
• Potentiometric probes are important tools for studying these processes
• These are fast-response probes that operate by means of change in their electronic structure, and consequently their fluorescence properties, in response to change in surrounding electric field Their optical response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells and intact brains However, magnitude of their potential-dependent fluorescence change is often small; fast-response probes typically show a 2-10% fluorescence change per 100mV Furthermore, these dyes display potential-dependent shift in their excitation spectra, thus permitting quantitation of membrane potential using excitation ratio measurements Zwitterionic molecule, exhibits most consistent potentiometric response in different cell and tissue types Ex/Em maxima bound to model phospholipid membranes are approximately 465/635nm (but spectral properties are highly dependent on environment) Nonfluorescent until bound to membranes Soluble in ethanol, DMSO, and DMF Dye is introduced into cells by direct addition of stock solution to cell culture medium or by using by retrograde labeling Fast-response probe, suitable for detecting submillisecond membrane potential changes Plasma membrane of cell typically has a transmembrane potential of approximately -70mV (negative inside) as a consequence of K+, Na+, and Cl- concentration gradients that are maintained by active transport processes Potentiometric probes offer an indirect method of detecting the translocation of these ions Cell Analysis, Cell Metabolism, Cell Structure, Cell Viability, Proliferation and Function, Membranes (General) and Lipids Order Info Shipping Condition: Room temperature