Molecular Probes™ alamarBlue™ Cell Viability Reagent

Description

• alamarBlue reagent is a redox indicator that yields a colorimetric change and a fluorescent signal in response to metabolic activity
• It is a proven safe and non-toxic dye used for quantitative analysis of cell viability and cell proliferation, for cytokine bioassays and in vitro cytotoxicity studies
• Applications: Cell proliferation determinations —alamarBlue reagent is water soluble, stable in culture, and non-toxic Cell viability assays, including cytotoxicity —metabolic activity and dye generation changes in proportion to altered viability and cell damage Cytokine assays —measure cytokine-induced proliferation, recover and expand cells at the end of the study if desired alamarBlue reagent as a cell viability assay reagent The alamarBlue assay is based on the ability of metabolically active cells to convert the reagent into a fluorescent and colorimetric indicator
• Damaged and non-viable cells have lower innate metabolic activity, and generate a proportionally lower signal
• The dye itself has low toxicity, and is generally safe for cells
• A safe, convenient, and versatile assay alamarBlue reagent is an indigo-colored liquid that has been shown to effectively measure innate metabolic activity in animal, fungal and bacterial cells
• Since alamarBlue reagent is non-toxic, the effector cells may be recovered for further analysis or cell expansion at the end of a study
• alamarBlue reagent is water-soluble, saving time otherwise spend on washing and extraction steps
• The homogeneous assay format of this kit is fast—add the single reagent directly to cells in suspension or to attached cells in full medium
• The protocol is readily amenable to automation and high-throughput assays
• The ability to detect the alamarBlue indicator by either fluorescence or spectroscopy provides compatibility with flow cytometry, plate readers and microscopy
• Experimental detection of alamarBlue reagent Fluorometry: by excitation at 530–560 nm and emission at 590 nm Spectrophotometry: by absorbance measurements at 570 nm and 600 nm