Gibco™ MDR1 Vesicular Transport Assay Reagent Set

Description

• The MDR1 Vesicular Transport Assay Reagent Set is a ready-to-use kit containing all reagents needed for vesicular transport assay except for the vesicles themselves
• The reagent set can be used for vesicular transport assay using radioisotope-labeled or non-labeled compound
• This kit has enough material to perform 100 vesicular transport assays
• Use The MDR1 Vesicular Transport Assay Reagent Set to: Investigate the transporter interactions of your drug candidates Assess potential for transporter-mediated drug-drug interactions Obtain high quality results with a large signal to noise ratios Concept Behind ABC Transporter Vesicles ABC transporter vesicles are easy-to-use, efficient reagents for early assessment of a drug candidate's substrate and drug interaction potential
• While ABC transporters typically mediate the export of substrates out of cells, transporters expressed on these inside-out vesicles import substrates into the vesicles
• It is therefore possible to quantitatively evaluate transport activity for your compound by determining the amount incorporated into the vesicles
• Clear and Reliable Results Prepared from Sf9 cells which have been engineered to over-express specific ABC transporters, these 'inside-out' vesicles provide high levels of transporter activity with low background, giving you a clear signal if your compound is a substrate or inhibitor of a specific efflux transporter
• Kit Compatibility Our MDR1 Vesicular Transport Assay Reagent Set is designed for use with MDR1 Vesicles
• The 100 vesicular transport assays that can be performed with this kit will require two MDR1 Vesicle products.