Invitrogen™ Image-iT™ Red Hypoxia Reagent

Description

• It is non-fluorescent when live cells are in an environment with normal oxygen concentrations and becomes fluorescent when oxygen levels are decreased
• Image-iT™ Red Hypoxia Reagent is a real-time oxygen detector, with a fluoregenic response that reverses when the cells return to normal oxygen levels
• Features of Image-iT™ Red Hypoxia Reagent include: • Measures hypoxia in live cells by fluorescing in low oxygen environments • Real-time oxygen detector, with reversible fluoregenic response • Easy to use—just add to cell culture media and image Image-iT™ Red Hypoxia Reagent is a fluorogenic compound that becomes fluorescent in environments with low oxygen concentrations, and it is live cell permeable
• These properties makes it a highly useful tool for detecting cells and tissue under hypoxic conditions
• Image-iT™ Red Hypoxia Reagent is a very sensitive oxygen detector
• Unlike pimonidazole adducts that respond only to very low oxygen levels, Image-iT™ Hypoxia Reagent begins to fluoresce when atmospheric oxygen levels are less than 5%
• It responds quickly to such environments, and the fluorogenic response reverses if oxygen concentrations improve
• These properties make Image-iT™ Red Hypoxia Reagent an ideal tool for detecting hypoxic conditions around tumors, 3D cultures, spheroids, neurons, etc
• It can be used to detect tumors in small animals, and its fluorogenic properties have been shown to corresponds with increased Hif 1α expression and translocation in hypoxic environments (1)
• 1
• Zhang, S., Hosaka, M., Yoshihara, T., Negishi, K., Iida, Y., Tobita, S., & Takeuchi, T
• (2010) Phosphorescent light–emitting iridium complexes serve as a hypoxia-sensing probe for tumor imaging in living animals
• Cancer Research 70(11), 4490-4498.