Invitrogen™ MitoSOX™ Mitochondrial Superoxide Indicators, for live-cell imaging

Description

• MitoSOX Green and MitoSOX Red superoxide indicators are novel fluorogenic dyes specifically targeted to mitochondria in live cells
• Oxidation of the MitoSOX reagent by mitochondrial superoxide produces bright green or red fluorescence
• Features of these indicators include: • Readily oxidized by superoxide but not by other ROS- or RNS-generating systems • Use for live cell imaging • Rapidly and selectively targeted to the mitochondria • MitoSOX Green indicator: absorption/emission maxima of ∼488/510 nm (traditional FITC/GFP filter set) • MitoSOX Red indicator: absorption/emission maxima of ∼396/610 nm* (custom filter set recommended) *Note that two excitation peaks may be observed for MitoSOX Red indicator, as it is excited at both 510 nm and 396 nm
• While 510 nm will excite the superoxide oxidation product, it can also excite non-specific products, so we recommend using a 396-nm excitation for more selective detection of mito superoxide when using MitoSOX Red indicator (1)
• MitoSOX Green indicator is offered as one vial or a pack of five vials
• MitoSOX Red indicator is offered as one vial or a pack of ten vials
• A variety pack of one vial each of MitoSOX Red and MitSOX Green indicators is also available
• Consult user Manual for solubility instructions
• Quick, easy detection of mitochondrial superoxide in live cells The production of superoxide by mitochondria can be visualized in fluorescence microscopy using MitoSOX superoxide indicators
• They permeate live cells where they selectively target mitochondria
• They are rapidly oxidized by superoxide but not by other reactive oxygen species (ROS) and reactive nitrogen species (RNS)
• The oxidized product is highly fluorescent
• MitoSOX indicators may be used to distinguish artifacts of isolated mitochondrial preparations from direct measurements of superoxide generated in the mitochondria of live cells
• They may also provide a valuable tool in the research of agents that modulate oxidative stress in various pathologies
• In addition, these indicators have been used in metabolic flux assays using high-content instruments (2)
• MitoSOX indicators have also been used to detect mitochondrial superoxide using flow cytometery (3)
• References 1: Robinson, Kristine M., et al
• Selective fluorescent imaging of superoxide in vivo using ethidium-based probes
• Proceedings of the National Academy of Sciences 103.41 (2006): 15038-15043
• 2: Little, Andrew Charles, et al
• High-content fluorescence imaging with the metabolic flux assay reveals insights into mitochondrial properties and functions
• Communications biology 3.1 (2020): 1-10
• 3: Kauffman, Megan E
• et al
• MitoSOX-Based Flow Cytometry for Detecting Mitochondrial ROS
• Reactive oxygen species (Apex, N.C.) vol
• 2,5 (2016): 361-370.