MP092690301
MP Biomedicals™ FastCellular™ Senescence Detection Kit, SPiDER-ΒGal
Manufacturer: MP Biomedicals™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MP092690301-Each-of-1 | In Stock | ₹ 36,020.76 |
MP092690301 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 36,020.76
GST (18%): ₹ 6,483.737
Total Price: ₹ 42,504.497
Content And Storage
Store at 0 to 5°C with protection from light
For Use With (Application)
Cellular Senescence Detection
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Description
- Cellular senescence is a phenomenon by which normal cells cease to divide and enter a state of irreversible growth arrest
- It is one of the most fundamental aspects of cellular behavior and generally believed to be associated with tumor suppressive mechanisms and an underlying cause of aging
- Senescence-associated Β-galactosidase (SA-Β-gal), which is overexpressed in senescent cells, has been widely used as a marker of cellular senescence
- Although X-gal is a well-known reagent used to detect SA-Β-gal, X-gal has the following disadvantages: 1) requirement of fixed cells due to poor cell-permeability, 2) low quantitative capability because of the difficulty in visually differentiating stained vs
- non-stained cells 3) requirement for long stain times
- To conquer the disadvantages inherent in using X-gal, we utilize a new reagent and method for the detection of cellular senescence
- SPiDER-ΒGal offers increased sensitivity in detecting SA-Β-gal due to increased cell permeability and retention inside cells
- Compared to the X-gal method, the FastCellular Senescence Detection Kit can be used for both living and fixed cells, because we provide Bafilomycin A1 to specifically inhibit endogenous Β-galactosidase activity in living cells and thus increase the detection specificity for living cells
- For fixed cells, SA-Β-gal is detectable by using SPiDER-ΒGal and Mcllvaine buffer (pH 6.0, please refer to the protocol for fixed cell assays)
- Since SPiDER-ΒGal emits strong and stable fluorescence after the reaction with SA-Β-gal, it can be applied to quantitative analysis by flow cytometry
- In addition, the staining time is only 30 mins
- Kit includes a 6-well plate with 1 unit of SPiDER-ΒGal and 1 unit of Bafilomycin A1 Benefits of using FastCellular Senescence Detection Kit: Higher sensitivity due to a new fluorogenic detection probe Applicable for living cells and fixed tissues Ability to quantify SA-Β-gal in cellular senescence assay Short staining time (30 min)
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Content And Storage: Store at 0 to 5°C with protection from light
For Use With (Application): Cellular Senescence Detection
Content And Storage:
Store at 0 to 5°C with protection from light
For Use With (Application):
Cellular Senescence Detection
Content And Storage: 15 to 30°C, desiccate, store under nitrogen
For Use With (Application): Cryopreservation
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Content And Storage: 15 to 30°C, desiccate, store under nitrogen
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Content And Storage: Stored at 2 to 8°C
For Use With (Application): Cryopreservation
Content And Storage:
Stored at 2 to 8°C
For Use With (Application):
Cryopreservation