Invitrogen™ Oregon Green™ 488 BAPTA-1, AM, cell permeant - Special Packaging

Description

• Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+
• They have uses in many calcium signaling investigations, including measuring intracellular Ca 2+ , following Ca 2+ influx and release, and multiphoton excitation imaging of Ca 2+ in living tissues
• Fluorescence signal from these cells is generally measured using fluorescence microscopy, fluorescence microplate assays, or flow cytometry Label (Ex/Em): Oregon Green 488 BAPTA-1 (494/523nm) Fluorescence intensity increase upon binding Ca 2+ : approximately 14 fold Exhibit fluorescence increase upon binding Ca 2+ with little shift in wavelength These probes are excited by visible light, and because energy required for excitation is low, potential for cellular photodamage is reduced Commonly used laser-based instruments (i.e., confocal laser scanning microscopes) are able to efficiently excite these indicators, and their emissions are in regions of spectrum where cellular autofluorescence and scattering backgrounds are often less of problem Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Cell-Based Second Messenger Assays, Drug Discovery and Development, G-Protein Coupled Receptor Biology, Ionic Homeostasis and Signaling, Target and Lead Identification and Validation Order Info Shipping Condition: Room temperature