Thermo Scientific™ Peroxidase Suppressor

Description

• Thermo Scientific™ Pierce Peroxidase Suppressor is a stable, easy-to-use inhibitor of endogenous peroxidase activity from cells and tissues to reduce background signal in HRP-based immunohistochemistry applications
• The immunoperoxidase method is a commonly used detection method for immunostaining of cells and tissues
• However, the analysis often is complicated by the presence of endogenous peroxidase and “pseudoperoxidase” activity in the cells and tissues
• This presence results in nonspecific staining when a substrate such as diaminobenzidine (DAB) is present
• Nonspecific staining is difficult to distinguish from the immunochemical marker, making it difficult to distinguish the final location of the antigen of interest
• The typical method of inhibiting endogenous peroxidase activity involves hydrogen peroxide or hydrogen peroxide in methanol
• The peroxidase suppressor inhibits peroxidase activity even more effectively than the method using hydrogen peroxide in methanol
• Plus, the Peroxidase Suppressor does not affect antibody activity
• Peroxidase Suppressor also can be used on blots of whole cell supernatant prior to the addition of the horseradish peroxidase (HRP) marker
• Highlights: Stable One component Ready-to-use Effectively inhibits peroxidase activity without interfering with antigen-antibody interactions