Invitrogen™ Rhod-5N, Tripotassium Salt, cell impermeant

Description

• Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+
• They have uses in many calcium signaling investigations, including measuring Ca 2+ in cells and tissues that have high levels of autofluorescence and also for detecting Ca 2+ release generated by photoreceptors and photoactivatable chelators
• Fluorescence signal from these cells is generally measured using fluorescence microscopy Fluorescence intensity increase upon binding Ca 2+ : >100 fold K d for Ca 2+ in absence of Mg 2+ , in buffer: approximately 320μM Exhibit fluorescence increase upon binding Ca 2+ with little shift in wavelength In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn 2+ , Zn 2+ , Pb 2+ ) with substantially higher affinity than Ca 2+ Perturbations to calcium measurements caused by presence of these ions can be controlled using heavy metal-selective chelator TPEN Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling Order Info Shipping Condition: Room temperature