Invitrogen™ SYTOX™ Deep Red Nucleic Acid Stain, for fixed/dead cells

Description

• With minimal cytoplasmic staining, SYTOX Deep Red stain is particularly useful in immunocytochemistry (ICC), immunohistochemistry (IHC), and immunofluorescence (IF) experiments for staining of nuclei of fixed cells
• After a brief incubation with SYTOX Deep Red stain, the nucleic acids of dead or fixed cells fluoresce a bright, deep red/far red and can be detected with a CY5/Deep Red standard filter set or laser configuration
• Features of SYTOX Deep Red Nucleic Acid Stain include: • Impermeant to live cells; permeant to dead or fixed cells • Excitation/emission peak: 660/682 nm • Use with Cy5 traditional filter set or 647 laser line • Works with mammalian cells in mono layer or 3D cell culture, animal tissue, and bacteria • Detectable with fluorescence micro¬scopes, fluorimeters, fluorescence microplate readers, and flow cytometers SYTOX Deep Red Nucleic Acid Stain is multiplexable with blue, green, orange, red, and near IR fluorophores, when compatible fluorescent filter/laser configurations are used
• SYTOX Deep Red stain shows increased fluorescence with increasing concentrations of dsDNA, but does not show much affinity for RNA or ssDNA
• It has a bright initial signal and excellent photo stability in a typical imaging experiment
• These properties make SYTOX Deep Red stain a simple and quantitative single-step dead/fixed cell nucleus-labeling dye for use with fluorescence micro¬scopes, fluorimeters, fluorescence microplate readers, and flow cytometers
• SYTOX Deep Red Nucleic Acid Stain had been used successfully in mono-layer cells, thin-sliced tissue, thick-sliced tissue, 3D cell cultures/spheroids, and bacteria
• It is supplied at a 1 mM concentration in DMSO (∼2000X concentration)
• A concentration of 0.5 μM (1X) is recommended for most applications and cell types
• In fluorescent imaging of various mammalian cells, a concentration range of 2.5 μM to 0.25 μM has been used
• For viability testing by flow cytometry, a concentration of 1 μM to 10 nM has been used for Jurkat cells and a range of 4 μM to 0.5 μM has been used with E
• coli.