Invitrogen™ Zymosan AS. cerevisiaeBioParticles™, fluorescein conjugate

Description

• Fluorescence of fluorescein-labeled BioParticles conjugates, partially quenched in acidic environments, fluorescence of the Alexa Fluor, BODIPY™ FL, tetramethylrhodamine and Texas Red™ dye conjugates is uniformly intense over the pH range from 3 to 10 Label (Ex/Em): Fluorescein (approx
• 494/518nm) Particle: Zymosan ( S
• cerevisiae ) Provided as lyophilized powders Approximately 3 x 10 8 E
• coli or S
• aureus particles per mg solid and approximately 2 x 10 7 zymosan particles per mg solid Conjugates can be reconstituted in the buffer of choice for use in phagocytosis assays Fluorescence of BioParticles conjugates that are bound to surface of cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers Addition to cellular applications, fluorescent BioParticles conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants Small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection Order Info Shipping Condition: Room Temperature