Invitrogen™ EVOS™ 10X Objective, achromat, LWD, phase-contrast, 0.25NA/7.45WD

Description

• This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates
• This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments
• All EVOS™ objectives offer outstanding optical performance from visible light to near infrared light
• The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications
• Additional characteristics of this EVOS™ objective: • Magnification: 10X • Numerical Aperture: 0.25 • Working Distance: 7.45 mm Image quality Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation
• Image quality is crucial to experimental success and a requirement for publication; EVOS™ objectives afford that quality across the visible spectrum to near infrared light
• This performance results from years of lens manufacture perfection
• EVOS™ objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements
• Objective classes Achromat objectives are perfect for general applications, with standard correction of color and focus
• Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging
• The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives
• Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging
• Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated
• They are recommended for the most demanding applications, particularly at magnifications of 60x and above
• Apochromatic objectives are the best choice for the capture of color images in white light
• Brightfield contrast versus phase contrast objectives Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light
• A higher density area in a sample will absorb more light, thus increasing contrast in those areas
• Phase contrast objectives are most useful for hard to see, translucent specimens
• This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast)
• Long working distance versus coverslip-corrected objectives Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm
• This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc
• Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm)
• These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.