Invitrogen™ Countess™ 3 Standard Slide

Description

• The Countess 3 Standard Slide is a ready-to-use and reusable slide used as a reference for cell counting, viability, and fluorescence on the Countess automated cell counters
• This slide is optimized for ensuring accurate performance in brightfield cell counting applications when using Countess II, Countess II FL, Countess 3, and Countess 3 FL automated cell counters
• The permanently mounted microspheres on the Countess 3 Standard Slide enable repeatable counts to be easily achieved over the life of the slide, with variability only encountered upon changing instrument gating settings
• The Countess 3 Standard Slide is supplied ready-to-use and does not require any special handling or sample preparation, thus eliminating the major source of count-to-count variability
• This makes it an ideal reference slide that can be used to achieve consistent and repeatable cell counts and viability measurements across days or between Countess 3 instruments
• The slide can also be used for demonstrating how to use the Countess automated cell counters for both brightfield and fluorescence cell counting
• Features of the Countess 3 Standard Slide include: Accurate —ready-to-use slide helps eliminate the error involved in creating standard solutions and dilution series Flexible —optimized for use in Countess II, Countess II FL, Countess 3, and Countess 3 FL automated cell counters Fast —supplied as a pre-loaded, immobilized, bead suspension; no preparation or mixing required Convenient —one standard slide for both brightfield and fluorescence cell counting applications Immobilized microspheres mimic cell counting samples The Countess 3 Standard Slide contains 15 μm microspheres that are permanently mounted in a cell counting chamber at a concentration of 0.5–1.5 x 10 6 /mL
• This unique, inert, immobilized bead mixture mimics the appearance of a 1:1 mixture of live and dead cells stained with trypan blue under brightfield illumination, which is the most common cell counting analysis method
• The permanently mounted microspheres provide a stable sample that can be used repeatedly for verification of instrument performance
• Please note that while the exact bead concentration for an individual slide is not specified, the bead concentration for each slide will fall in the 0.5–1.5 x 10 6 /mL range and remain consistent over time
• Fluorescent microspheres enable fluorescence cell counting The Countess 3 Standard Slide offers further utility by containing two populations of fluorescent beads that cover the fluorescence spectrum from blue to far red, thereby allowing analysis with a variety of EVOS light cube combinations available to Countess II FL and Countess 3 FL counter users
• When viewed on the Countess 3 FL counter, the 15 μm microspheres are also fluorescent in a 1:1 mixture, providing approximately 50% detection with a DAPI or GFP light cube and approximately 50% detection with an RFP, Texas Red, or Cy5 light cube
• The fluorescent microspheres enable users to perform or demonstrate fluorescence-based cell counting with the Countess 3 Standard Slide
• Stable standard for routine performance verification The Countess 3 Standard Slide provides a stable sample containing microspheres that are visible in both brightfield and fluorescence channels and mimic a mixed population of live and dead cells
• This allows users to easily perform routine verification of instrument performance for automated cell counting and demonstrate instrument usage
• The immobilized microspheres eliminate sample variability, enabling reliable ongoing quality control.