Invitrogen™ CytoVista™ 3D Cell Culture Clearing/Staining Kit

Description

• Clearing process enables sharp and bright 3D fluorescent imaging for depths up to 1000 μm
• The kit includes CytoVista™ 3D Cell Culture Clearing Reagent, penetration buffer, wash buffer, blocking buffer, and antibody dilution buffer
• Features of the CytoVista™ family of tissue and 3D cell culture clearing reagents include: • Rapid clearing of fluorescently labeled tissue for 3D imaging • Minimal shrinkage, expansion, or other morphological changes to tissue • Simple workflow does not require any special instruments or equipment and is easily adapted to IHC • Compatible with most fluorophores, including dyes and fluorescent proteins The CytoVista™ tissue and 3D cell culture clearing workflow clears tissue or 3D cultured cells with minimal changes to morphology or the sensitivity of detection with almost any fluorophore
• With an easy-to-use protocol, whole mouse brain (8-mm section) can be cleared in 24 hours, or a 1-mm section in 2 hours, without the use of any special instruments or equipment
• The CytoVista™ clearing workflow is compatible with most fluorophores, including fluorescent proteins that are detected with common fluorescent imaging instruments, such as wide-field microscopes, high content analyzers, confocal microscopes, and lightsheet microscopes
• For first time users, we recommend the CytoVista™ Tissue Clearing/Staining Kit (Cat No V11324) or CytoVista™ 3D Cell Culture Clearing/Staining Kit (Cat No
• V11325)
• These kits contain all necessary reagents for both clearing and IHC staining
• CytoVista™ clearing technology sufficiently clears tissue for 3D fluorescent imaging, but unlike other clearing techniques does not significantly changes the tissue morphology
• Minimal morphological changes, such as shrinkage and contraction, have been observed
• For precious samples, the clearing can be reversed, and the tissue can be processed for histology studies, like H&E staining
• For tissues that are easier to clear, like brain, lung, intestine, skin, and tissues of less than 250-μm thickness, treatment with CytoVista™ Tissue Clearing Reagent is sufficient for 3D imaging
• However, tissues of more than 250-μm thickness or that are hard to clear like kidney, liver, heart, and placenta will require an initial treatment with CytoVista™ Tissue Clearing Reagent followed by treatment with CytoVista™ Tissue Clearing Enhancer
• CytoVista™ 3D Cell Culture Clearing Reagent is recommended for spheroids or organoids up to 1 mm in thickness
• For hard tissues like bone, cartilage, and connective tissue, CytoVista™ Tissue Clearing Reagent is not recommended.