Invitrogen™ eBioscience™ Human Intracellular (cytoplasmic/cytokine) Protein Flow Cytometry Workflow Kit

Description

• Description Kit contains all of the normal buffers, blockers, and beads to complete a standard flow cytometry experiment for staining intracellular cytoplasmic and cytokine targets in human cell suspensions When combined with experiment-specific antibodies, the reagents within this kit have been optimized to work together to detect human intracellular cytopasmic and cytokine targets by flow cytometry Effective compensation controls Fc receptor blocking Non-antibody, non-protein based blocking solution for reducing background staining from fluorophores Antibody binding buffer minimizes non-specific binding of antibodies Fixation buffer for storage of stained samples Activating cocktail to induce cytokine production Kit includes UltraComp eBeads™ Plus Compensation Beads (01-3333-41) Fc Receptor Binding Inhibitor Polyclonal Antibody, eBioscience (14-9161-71) CellBlox™ Blocking Buffer (B001T06F01) eBioscience Intracellular Fixation & Permeabilization Buffer Set (88-8824-00) eBioscience™ Flow Cytometry Staining Buffer (00-4222-57) eBioscience Cell Stimulation Cocktail plus protein transport inhibitors, 500X (00-4975-93)