Invitrogen™ FIX & PERM™ Cell Permeabilization Kit

Description

• The FIX & PERM™ Cell Permeabilization Kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing access of antibody to intracellular targets without sacrificing scatter characteristics
• The FIX & PERM™ Cell Permeabilization Kit is: Flexible—compatible with multiple sample types and cellular antigens Robust—scatter pattern of cells unaffected Efficient—simultaneous detection of intracellular and surface markers View a selection guide for all available FIX & PERM™ products and products related to sample preparation (including cell lysis and preservation solutions, blocking reagents, and information about the use of Dynabeads™ cell separation products for cell isolation)
• The FIX & PERM™ formula was specifically designed to reduce background staining and allow simultaneous addition of fluorochrome-labeled antibodies during the permeabilization step.The FIX & PERM™ Cell Permeabilization Kit is suitable for various biological samples and has been validated for use with cell lines, whole blood, and bone marrow
• The FIX & PERM™ kit has been shown to accurately identify previously undetectable intracellular markers
• Unlike other fixation and permeabilization reagents available on the market, the FIX & PERM™ staining protocol leaves the morphological scatter characteristics of the cells intact
• How It Works The FIX & PERM™ Cell Permeabilization Kit contains 2 reagents: Fixation Medium (Medium A) and Permeabilization Medium (Medium B)
• Cells are incubated for 15 minutes at room temperature with Fixation Medium, washed, and then incubated with the Permeabilization Medium and fluorochrome-labeled antibody
• Fixation Medium and Permeabilization Medium are also available separately (Cat
• Nos
• GAS001S100 & GAS002S100).