Invitrogen™ Dynabeads™ Protein A for Immunoprecipitation

Description

• Dynabeads Protein A beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface
• Dynabeads Protein A beads provide a great alternative to Sepharose or agarose resins for immunoprecipitation (IP) combined with your own antibody, and both manual and automated protocols are available
• Benefits of Dynabeads Protein A beads include: • Fast and easy —IP in less than 40 minutes with no columns, centrifugations, or time-consuming pre-clearing required • High performance —high target protein yield with low antibody consumption • Low background ­—very low non-specific binding with a high signal-to-noise ratio • High reproducibility —uniform beads ensure the most consistent results • Automation ready —automation protocols available with the KingFisher Sample Purification instruments Gentle separation causes minimal physical stress to proteins The magnetic separation technology utilized by Dynabeads Protein A is rapid and gentle, causing minimal physical stress to target proteins
• This permits the isolation and concentration of labile composites that might otherwise dissociate or be damaged by proteases during long incubation times
• Native protein conformation and large protein complexes are preserved
• Binding strength and antibody binding capacity Dynabeads Protein A beads allow for the isolation of most mammalian immunoglobulins (Ig)
• The amount of Ig captured depends on the concentration of Ig in the starting sample, and the type and source of the Ig
• 100 μL of Dynabeads Protein A will isolate approximately 25–30 μg human IgG from a sample containing 20–200 μg IgG/mL
• Predominantly Fc-binding allows optimal Ig orientation
• Dynabeads magnetic beads are not porous in nature such as Sepharose or agarose resins
• Thus, the antibodies bind to the smooth outer surface of the beads and are available to bind to the target protein
• This translates to a low antibody consumption while obtaining a high yield of the target protein
• The smooth bead surface is also the reason for the low non-specific binding that Dynabeads magnetic beads are known for
• The recombinant protein A on the beads does not contain albumin binding sites, thus albumin is not co-purified during the procedure
• Dynabeads immunoprecipitation protocol is fast and easy The manual IP protocol is simple and can be performed in less than 40 minutes with 3 easy bind-incubate-elute steps
• The procedure does not require any pre-clearing due to the low non-specific binding and uses very little antibody while still obtaining a high IP yield, thus saving both time and cost per IP
• The antibody-coated beads can be used in a variety of IP applications such as Co-IP, chromatin IP (ChIP/ChIP sequencing), RNA IP (RIP, RIP sequencing), small-scale IgG purification, and protein purification
• The isolated pure target protein can be used in downstream western blot, mass spectrometry analysis, sequencing, etc
• Automation-ready for use with KingFisher purification systems The 2.8-μm magnetic beads are ideal for high-throughput enrichment
• This process can be automated using any of our KingFisher sample purification systems Commercial supply Our manufacturing sites are ISO 13485-certified
• Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply
• If you are in the process of customizing Dynabeads Protein A beads in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or read more on our Dynabeads OEM information page
• Learn more about Dynabeads magnetic beads and KingFisher Sample Purification Systems: • Dynabeads Protein A beads are also available as a 'ready-to-go' kit with buffers included (Cat
• No
• 10006D) • See our immunoprecipitation selection table, data, and references • Dynabeads magnetic beads selection guide • See magnets for Dynabeads magnetic beads separations • KingFisher automation protocols • Watch a video about the KingFisher Flex instrument *Sepharose is a trademark of GE Healthcare Bio-Sciences AB
• Order Info Shipping Condition: Room Temperature