Invitrogen™ Exosome-Human CD9 Isolation Reagent (from cell culture)

Description

• Obtain a highly pure exosome subset with easy protocol scalability with Exosome-Human CD9 Isolation Reagent (from cell culture)
• Following isolation, the specific sub-population of exosomes, ECVs can be further studied using methods such as Western blotting, qPCR, or next generation sequencing
• Minimal hands-on time for specific exosome isolation Compatible with most downstream analysis Recent studies suggest that exosome/ECV population consists of several subsets, possibly with different functions Using immuno-affinity based Dynabeads magnetic separation technology, these subsets can be selectively isolated and studied Cell culture media must first be enriched for exosomes Can be done quickly and efficiently using Total Exosome Isolation Reagent (from cell culture media) or traditional ultracentrifugation Following enrichment, specific exosomes are isolated by binding to surface of magnetic beads In addition to their high-quality sensitivity, reproducibility, and stability, Dynabeads allows you to see samples during handling due to color of magnetic beads When a sample tube is placed in the magnet, bead-bound exosomes are pulled to side of tube, allowing for easy separation and buffer changes Sample and bead volume can be easily scaled according to your sample size or downstream applications Mix thoroughly to ensure that magnetic beads do not settle in the sample tube
• A mixer which provides both tilting and rotation for optimal mixing is recommended.