11-363-D
Invitrogen™ Dynabeads™ Regulatory CD4+/CD25+ T Cell Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 11-363-D-Each-of-1 | In Stock | ₹ 1,02,158.64 |
11-363-D - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,02,158.64
GST (18%): ₹ 18,388.555
Total Price: ₹ 1,20,547.195
Cell Type
T Cells (Regulatory)
Product Type
Cell Isolation Kit
No. of Cells
Processes ∼1 x 109cells total
Product Line
DETACHaBEAD™, DYNAL™, Dynabeads™, FlowComp™
Quantity
2 x 5 mL
Shipping Condition
Room Temperature
Target Species
Human
Content And Storage
Each Kit Contains:• 2 mL Antibody Mix Human CD4• 10 mL Depletion MyOne™ Dynabeads™• 5 mL Dynabeads™ CD25• 2 mL DETACHaBEAD™.These reagents are sufficient to process ∼1 x 109cells. Store components at 2°C to 8°C.
Isolation Technology
Depletion, then positive isolation
Output Viability
>95%
Purity or Quality Grade
Research Grade
Sample Type
PBMC
Starting Material Cell No.
1 x 108PBMCs per isolation
Related Products
Description
- Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3
- The CD4+ CD25- cell fraction can be used as effector cells in downstream inhibitory assays
- You can expect >95% purity (CD4+ CD25+ expression) and >80% Foxp3 expression
- Isolate bead- and antibody-free human CD4+ CD25+ Foxp3+ Treg cells • Treg cells can be expanded with Dynabeads™ CD3/CD28 Treg Expander and remain Foxp3+
- • Treg cells can be used in any downstream applications (e.g., suppression assays) Methodology The isolation procedure includes the following steps: • Negative isolation of untouched human CD4+ T-cells from PBMC
- • Positive isolation of CD25+ T-cells from the untouched CD4+ T-cell subset
- • Removal of beads from the CD4+CD25+ Treg cells with DETACHaBEAD™ Starting sample PBMC Order Info Shipping Condition: Room Temperature
Compare Similar Items
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Cell Type: T Cells (Regulatory)
Product Type: Cell Isolation Kit
No. of Cells: Processes ∼1 x 109cells total
Product Line: DETACHaBEAD™, DYNAL™, Dynabeads™, FlowComp™
Quantity: 2 x 5 mL
Shipping Condition: Room Temperature
Target Species: Human
Content And Storage: Each Kit Contains:• 2 mL Antibody Mix Human CD4• 10 mL Depletion MyOne™ Dynabeads™• 5 mL Dynabeads™ CD25• 2 mL DETACHaBEAD™.These reagents are sufficient to process ∼1 x 109cells. Store components at 2°C to 8°C.
Isolation Technology: Depletion, then positive isolation
Output Viability: >95%
Purity or Quality Grade: Research Grade
Sample Type: PBMC
Starting Material Cell No.: 1 x 108PBMCs per isolation
Cell Type:
T Cells (Regulatory)
Product Type:
Cell Isolation Kit
No. of Cells:
Processes ∼1 x 109cells total
Product Line:
DETACHaBEAD™, DYNAL™, Dynabeads™, FlowComp™
Quantity:
2 x 5 mL
Shipping Condition:
Room Temperature
Target Species:
Human
Content And Storage:
Each Kit Contains:• 2 mL Antibody Mix Human CD4• 10 mL Depletion MyOne™ Dynabeads™• 5 mL Dynabeads™ CD25• 2 mL DETACHaBEAD™.These reagents are sufficient to process ∼1 x 109cells. Store components at 2°C to 8°C.
Isolation Technology:
Depletion, then positive isolation
Output Viability:
>95%
Purity or Quality Grade:
Research Grade
Sample Type:
PBMC
Starting Material Cell No.:
1 x 108PBMCs per isolation
Cell Type: Monocytes
Product Type: Superparamagnetic Beads
No. of Cells: 2 x 109PBMC, 80 mL whole blood, or 160 mL buffy coat
Product Line: FlowComp™
Quantity: 2 x 3 mL
Shipping Condition: Room Temperature
Target Species: Human
Content And Storage: The Kit Contains:• 2 x 3 mL FlowComp™ Dynabeads™• 1 mL FlowComp™ Human CD14 Antibody• 2 x 20 mL FlowComp™ Release Buffer.Store at: 2°C to 8°C.
Isolation Technology: Positive isolation - cells are left bead-free after isolation
Output Viability: __
Purity or Quality Grade: Research Grade, 99%
Sample Type: PBMC, Buffy Coat, Blood
Starting Material Cell No.: __
Cell Type:
Monocytes
Product Type:
Superparamagnetic Beads
No. of Cells:
2 x 109PBMC, 80 mL whole blood, or 160 mL buffy coat
Product Line:
FlowComp™
Quantity:
2 x 3 mL
Shipping Condition:
Room Temperature
Target Species:
Human
Content And Storage:
The Kit Contains:• 2 x 3 mL FlowComp™ Dynabeads™• 1 mL FlowComp™ Human CD14 Antibody• 2 x 20 mL FlowComp™ Release Buffer.Store at: 2°C to 8°C.
Isolation Technology:
Positive isolation - cells are left bead-free after isolation
Output Viability:
__
Purity or Quality Grade:
Research Grade, 99%
Sample Type:
PBMC, Buffy Coat, Blood
Starting Material Cell No.:
__
Cell Type: Primary Rat Astrocytes
Product Type: MEM (Minimum Essential Medium)
No. of Cells: __
Product Line: Gibco™
Quantity: 500 mL
Shipping Condition: Room Temperature
Target Species: __
Content And Storage: Storage conditions: 2-8°C. Protect from lightShipping conditions: AmbientShelf life: 12 months from date of manufacture
Isolation Technology: __
Output Viability: __
Purity or Quality Grade: __
Sample Type: __
Starting Material Cell No.: __
Cell Type:
Primary Rat Astrocytes
Product Type:
MEM (Minimum Essential Medium)
No. of Cells:
__
Product Line:
Gibco™
Quantity:
500 mL
Shipping Condition:
Room Temperature
Target Species:
__
Content And Storage:
Storage conditions: 2-8°C. Protect from lightShipping conditions: AmbientShelf life: 12 months from date of manufacture
Isolation Technology:
__
Output Viability:
__
Purity or Quality Grade:
__
Sample Type:
__
Starting Material Cell No.:
__
Cell Type: __
Product Type: Taylor Universal Test Tube
No. of Cells: __
Product Line: __
Quantity: __
Shipping Condition: __
Target Species: __
Content And Storage: __
Isolation Technology: __
Output Viability: __
Purity or Quality Grade: __
Sample Type: __
Starting Material Cell No.: __
Cell Type:
__
Product Type:
Taylor Universal Test Tube
No. of Cells:
__
Product Line:
__
Quantity:
__
Shipping Condition:
__
Target Species:
__
Content And Storage:
__
Isolation Technology:
__
Output Viability:
__
Purity or Quality Grade:
__
Sample Type:
__
Starting Material Cell No.:
__