Invitrogen™ Dynabeads™ Antibody Coupling Kit

Description

• The Dynabeads™ Antibody Coupling Kit is intended for the coupling of an antibody or protein to the surface of Dynabeads™ M-270 Epoxy beads
• The coupled beads and supplied buffers can then be used for experiments such as immunoassays, immunoprecipitation (IP), Co-IP of protein complexes, chromatin IP (ChIP), RNA-IP (RIP), cell isolation, etc
• The Dynabeads Epoxy beads in this kit do not contain Tween™ detergent making them suitable for mass spectrometry (MS) analysis
• The kit includes the buffers needed for efficient antibody coupling and washing steps
• Note that the SB buffer supplied in the kit does contain Tween™ detergent, which means the buffer will need to be replaced with standard TBS or PBS buffer if the kit is to be used for MS
• Benefits of Dynabeads Antibody Coupling Kit include: • Convenience —contains optimized buffers and epoxy-activated Dynabeads magnetic beads • Efficiency­ —easy-to-use protocol with minimal hands-on time • Flexibility­ —allows coupling of any antibody of your choice • Robustness —covalent antibody coupling to the beads avoids co-elution of the antibody with the target proteins Efficient and easy coupling With this kit antibodies are covalently coupled to Dynabeads M-270 Epoxy beads, minimizing the risk of bound antibodies contaminating the final eluate
• Dynabeads M-270 Epoxy beads exhibit extremely low background binding and do not require blocking before use
• Other proteins such as lectins and enzymes may also be coupled using the same beads, buffers, and protocol (depending on the stability and functionality of the protein/ligand you are using)
• Magnetic bead-based separation offers easy handling Captured proteins and protein complexes are easily separated, washed, and eluted using a DynaMag magnet due to the magnetic properties of the Dynabeads M-270 Epoxy beads
• The supplied buffers are optimized to give consistent and reliable results
• In addition to Dynabeads M-270 Epoxy beads, the kit includes five different binding, washing, and elution buffers
• Target specific antibody is required for coupling, but not included
• Automation-ready for use with KingFisher purification systems The 2.8-μm magnetic beads are ideal for high-throughput enrichment
• This process can be automated using any of our KingFisher sample purification systems or liquid handlers
• Commercial supply Our manufacturing sites are ISO 13485-certified
• Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply
• If you are in the process of customizing Dynabeads Antibody Coupling Kit in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or read more on our Dynabeads OEM page
• Order Info Shipping Condition: Room Temperature