Invitrogen™ DynaGreen™ Protein A Magnetic Beads

Description

• DynaGreen Protein A Magnetic Beads are intended for use in immunoprecipitation (IP) applications while also being an environmentally sustainable choice
• They are microplastic-free submicron magnetic beads that provide reproducible isolation of target proteins in a simple and efficient workflow, which can be performed manually or automated on KingFisher™ instruments
• Benefits of DynaGreen Protein A Magnetic Beads include: • High performance —reproducible IP with high-yield and low non-specific binding • Reduced environmental impact —non-microplastic bead core and holistic sustainable product design • Flexible —direct and indirect immunoprecipitation helps ensure isolation of non-abundant proteins • Automation ready —easy automation for high-throughput needs on the Kingfisher Sample Purification Systems Simple, gentle, and efficient manual separation DynaGreen Protein A Magnetic Beads are microplastic-free superparamagnetic beads with recombinant protein A (∼45 kDa) covalently coupled to the surface
• They were developed for convenient IP of target proteins when the IP antibody interacts strongly with Protein A (see subclass specificity in the manual)
• The submicron bead size (∼250 nm) provides low sedimentation and a large target capture surface area
• The method is a simple bind-incubate-elute protocol with no pre-clearing required
• This results in an efficient high-yield isolation of target protein by direct or indirect immunoprecipitation in less than 80 minutes
• The magnetic separation technology used by DynaGreen Protein A Magnetic Beads is rapid and gentle, thereby reducing physical stress to the target proteins
• Furthermore, the non-porous nature of the beads lowers the risk of antibodies being trapped and becoming inaccessible
• This helps to minimize the amount of antibody required to produce accurate, and reproducible results
• The yield and purity of the isolated target protein are highly compatible for use in western blotting and mass spectrometry
• The product manual below includes a list of required reagents, materials, and details of compatible buffer recipes
• Rapid and reproducible automated workflow The DynaGreen magnetic beads are ideal for high-throughput enrichment
• This process can be automated using any of our KingFisher sample purification systems or liquid handlers
• The automated protocol can be performed in just 40 minutes with up to 96 samples per run, which helps reduce hands-on time while maintaining the same reproducible high target yield and low non-specific binding compared to the manual protocol
• A certified choice for environmentally sustainable research Microplastic-free DynaGreen Protein A Magnetic Beads are designed for both high performance in immunoprecipitation workflows and lower environmental impact
• They provide exceptional purity and high yield of target proteins compared to other magnetic beads in both direct and indirect immunoprecipitation workflows
• At the same time, the product boasts an extensive list of third-party ACT-label-certified sustainable product design elements by incorporating principles of green chemistry and green engineering
• Choosing DynaGreen Protein A Magnetic Beads can help reduce the environmental impact of research without compromising on results
• Learn more about our DynaGreen magnetic beads and KingFisher Sample Purification Systems: • Alternative sustainable products: DynaGreen Protein A/G (Cat
• No
• 80106G) and DynaGreen Capture-Select anti-FC (multispecies) (Cat
• No
• 80107G) • DynaGreen magnetic beads sustainability • DynaMag magnets for magnetic bead separations • KingFisher automation protocols • KingFisher Flex instrument video