Molecular Probes™ FluoSpheres™ Size Kit #2, Carboxylate-modified Microspheres, yellow-green fluorescent (505/515), 2% solids, six sizes

Description

• Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene
• Our Molecular Probes™ FluoSpheres™ beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy
• This FluoSpheres™ Size Kit supplies 1 mL of each the 0.02, 0.1, 0.2, 0.5, 1.0, and 2.0μm sizes
• FluoSpheres™ Microsphere Specifications • Label (Ex/Em): Yellow-green fluorescent (505/515) • Nominal bead diameter: 0.02, 0.1, 0.2, 0.5, 1.0, 2.0μm • Coupling surface: Carboxylate • Solids: 2% Characteristics of the Various FluoSpheres™ Coupling Surfaces • Carboxylate-modified FluoSpheres™ beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• • Sulfate FluoSpheres™ beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• • Aldehyde-sulfate FluoSpheres™ beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions
• • Amine-modified FluoSpheres™ beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide
• Key Applications of Microspheres • Instrument calibration (flow cytometry, microscopy, HTS, HCS) • Flow testing (microfluidics, blood flow, water flow, and air flow) • Cell biology tracers (cell differentiation and cell tracing) • Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents) Choices for FluoSpheres™ Fluorescent Microspheres Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations: • Ten fluorescent colors • Ten nominal bead diameters: 0.02μm, 0.04μm, 0.1μm, 0.2μm, 0.5μm, 1.0μm, 2.0μm, 4.0μm, 10.0μm, and 15.0μm • Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine • Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum Choices for Unstained Microspheres We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications
• We’ll Make a Custom Microsphere Product for You We will prepare custom orders upon request
• For example, FluoSpheres™ beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications
• Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work
• We are ISO 13485:2000 certified
• For Research Use Only
• Not intended for animal or human therapeutic or diagnostic use.