LIF human

Description

• General description: Leukemia inhibitory factor (LIF) is a secreted pleiotropic glycoprotein which is a member of the gp130 signaling of interleukin-6 (IL-6) family cytokines.[1][2] This protein was originally identified as a protein that prevented the growth of a leukemic cell line.[3] Recombinant human LIF is a 19.6 kDa protein containing 180 amino acids residues including three disulfide bonds.
• Biochem/physiol Actions: Leukemia inhibitory factor (LIF) suppresses the differentiation of myoblasts, and inhibits the activation of caspase-3 and blocks the reduction in Bcl-xL (B-cell lymphoma-extra large) levels, thus, preventing doxorubicin-induced apoptosis. Along with transforming growth factor β2 (TGFβ2)/fibroblast growth factor 2 (FGF2), LIF regulates nephrogenesis. It facilitates tubular regeneration following acute kidney failure. It plays a crucial role in neuroprotective pathways induced by preconditioning-induced oxidative stress.[1] LIF is capable of maintaining the totipotency of embryonic stem cells.[3] It is also involved in controlling the differentiation of embryonic stem cells, hematopoietic and neuronal cells.[2]
• Physical form: Lyophilized with no additives
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.