DMEM/F-12
With HEPES, sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, sterile liquid, suitable for cell culture
Manufacturer: Sigma Aldrich
Synonym(S): DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 500 ML | D6421-500-ML | In Stock | ₹ 6,928.00 |
D6421 - 500 ML
In Stock
Quantity
1
Base Price: ₹ 6,928.00
GST (18%): ₹ 1,247.04
Total Price: ₹ 8,175.04
product name
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Level
400
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: 0.055 g/LL-glutamine: nophenol red: yesNaHCO3: yesHEPES: 15 mMglucose: 3.15 g/L
shipped in
ambient
storage temp.
2-8°C
Related Products
Description
- General description: A measured mixture of chemically defined ingredients makes up a basal medium, which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/F-12 is a completely defined synthetic medium, which is protein-free.[1]
- Application: DMEM:F12 is a 50:50 mixture of DMEM and Ham′s F12 media that has proven to be useful in a wide range of cell culture applications, especially when supplemented with fetal bovine serum (FBS). Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells.
- Reconstitution: Supplement with 0.365 g/L L-glutamine.
SAFETY INFORMATION
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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product name: Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Level: 400
sterility: sterile-filtered
form: liquid
technique(s): cell culture | mammalian: suitable
impurities: endotoxin, tested
components: sodium pyruvate: 0.055 g/LL-glutamine: nophenol red: yesNaHCO3: yesHEPES: 15 mMglucose: 3.15 g/L
shipped in: ambient
storage temp.: 2-8°C
product name: Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Level: 400
sterility: sterile-filtered
form: liquid
technique(s): cell culture | mammalian: suitable
impurities: endotoxin, tested
components: HEPES: noNaHCO3: yesphenol red: yessodium pyruvate: yesL-glutamine: yesglucose: high
shipped in: ambient
storage temp.: 2-8°C
product name: Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
Quality Level: 400
sterility: sterile-filtered
form: liquid
technique(s): cell culture | mammalian: suitable
impurities: endotoxin, tested
components: L-glutamine: nophenol red: noNaHCO3: yesHEPES: 15 mMsodium pyruvate: 0.055 g/Lglucose: 3.15 g/L
shipped in: ambient
storage temp.: 2-8°C
