Murashige and Skoog Basal Salt Mixture (MS)

Description

• General description: Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and Kinetin (cytokinin/cell division promoter).
• Application: Murashige and Skoog Basal Salt Mixture (MS) has been used to support the growth and regeneration of transformed tobacco cells.[1] It has also been used as a growth medium for germination of Arabidopsis thaliana (Col-0) seeds.
• Formula variant: With the macro- and micronutrients as described by Murashige and Skoog (1962).Media Formulation
• Quantity: Formulated to contain 4.3 grams of powder per liter of medium.
• Preparation Note: Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M5524) contains the macronutrients and micronutrients of the original classic formulation. It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.
• Other Notes: StoragePowdered media are extremely hygroscopic and must be protected from atmospheric moisture. If possible the entire contents of each package should be used immediately after opening.Store dry medium in a desiccator at 0-5 °C.Deterioration of powdered medium may be recognized by: 1) color change; 2) granulation, clumping, or particulate matter throughout the powder; 3) insolubility; 4) pH change; or 5) inability to promote growth when properly used.Media Preparation