C-12285
Human Cardiac Microvascular Endothelial Cells (HCMEC)
500,000 cryopreserved cells
Manufacturer: Sigma Aldrich
Synonym(S): Human endothelial cells
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 EACH | C-12285-1-EACH | In Stock | ₹ 1,33,455.30 |
C-12285 - 1 EACH
In Stock
Quantity
1
Base Price: ₹ 1,33,455.30
GST (18%): ₹ 24,021.954
Total Price: ₹ 1,57,477.254
biological source
human heart (ventricles)
packaging
pkg of 500,000 cells
morphology
( endothelial)
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
diabetes; hypertension; ischemia/reperfusion injury
shipped in
dry ice
storage temp.
−196°C
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Description
- General description: Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
- Cell Line Origin: Heart
- Application: Primary Human Cardiac Microvascular Endothelial Cells (HCMEC) are isolated from heart ventricles from a single donor and are provided in a cryopreserved format. The cells are routinely analyzed by immunofluorescent staining: they stain positive for CD31 and Willebrand factor and negative for smooth muscle alpha-actin. HCMEC intensively interact with cardiomyocytes and therefore have a characteristic phenotype different from other microvascular endothelial cells. HCMEC play an important role in the physiological regulation of coronary blood flow and capillary exchange. Medical conditions such as hypertrophy, ischemia, hypertension, and diabetes mellitus are associated with endothelial dysfunction.
- Quality: Rigid quality control tests are performed for each lot of Microvascular Endothelial Cells. They are tested for cell morphology and cell type specific markers, e.g. von Willebrand Factor (vWF), CD31 and Podoplanin using flow cytometric analyses. Growth performance is tested through multiple passages up to 15 population doublings (PD) without antibiotics or antimycotics. In addition, all cells have been tested forthe absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
- Warning: Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
- Subculture Routine: Click here for more information.
- Other Notes: Recommended Plating Density: 10000 - 20000 cells per cm2Passage After Thawing: P2Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15
- Recommended products: Recommended Primary Cell Culture Media:Link
- Disclaimer: RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
SAFETY INFORMATION
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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biological source: human heart (ventricles)
packaging: pkg of 500,000 cells
morphology: ( endothelial)
technique(s): cell culture | mammalian: suitable
relevant disease(s): diabetes; hypertension; ischemia/reperfusion injury
shipped in: dry ice
storage temp.: −196°C
biological source:
human heart (ventricles)
packaging:
pkg of 500,000 cells
morphology:
( endothelial)
technique(s):
cell culture | mammalian: suitable
relevant disease(s):
diabetes; hypertension; ischemia/reperfusion injury
shipped in:
dry ice
storage temp.:
−196°C
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relevant disease(s): __
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cell culture | mammalian: suitable
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biological source: human dermis
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cell culture | mammalian: suitable
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__
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dry ice
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−196°C