C-12302
Normal Human Dermal Fibroblasts (NHDF)
Adult, 500,000 cryopreserved cells
Manufacturer: Sigma Aldrich
Synonym(S): NHDF cells
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 EACH | C-12302-1-EACH | In Stock | ₹ 62,781.60 |
C-12302 - 1 EACH
In Stock
Quantity
1
Base Price: ₹ 62,781.60
GST (18%): ₹ 11,300.688
Total Price: ₹ 74,082.288
biological source
human dermis
packaging
pkg of 500,000 cells
morphology
(fibroblast)
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
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Description
- General description: Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
- Cell Line Origin: Skin
- Application: Primary Normal Human Dermal Fibroblasts (NHDF) are isolated form the dermis of adult skin (different locations) and are provided in a cryopreserved format. They can be used for wound healing studies and dermatological research to investigate diseases like scleroderma, fibrosarcoma, fibrosis, xeroderma pigmentosum, and histiocytoma. Moreover, fibroblasts are important for cancer research, tissue regeneration, and tissue engineering studies.Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available on request.
- Quality: Rigid quality control tests are performed for each lot of Human Fibroblasts. They are tested for cell morphology, adherence rate and cell viability. Flow cytometric analyses of the fibroblast specific antigen CD90 are performed for each lot. Growth performance is tested through multiple passages up to 10 or 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
- Warning: Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
- Subculture Routine: Click here for more information.
- Other Notes: Recommended Plating Density: 3500 - 7000 cells per cm2Passage After Thawing: P2Tested Markers: CD90 positiveGuaranteed population doublings: >15
- Recommended products: Recommended Primary Cell Culture Media:Link
- Disclaimer: RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
SAFETY INFORMATION
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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biological source: human dermis
packaging: pkg of 500,000 cells
morphology: (fibroblast)
technique(s): cell culture | mammalian: suitable
shipped in: dry ice
storage temp.: −196°C
biological source:
human dermis
packaging:
pkg of 500,000 cells
morphology:
(fibroblast)
technique(s):
cell culture | mammalian: suitable
shipped in:
dry ice
storage temp.:
−196°C
biological source: human lung
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morphology: (fibroblast)
technique(s): cell culture | mammalian: suitable
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packaging:
pkg of 500,000 cells
morphology:
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technique(s):
cell culture | mammalian: suitable
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−196°C
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packaging: pkg of 500,000 cells
morphology: (epidermal)
technique(s): cell culture | mammalian: suitable
shipped in: dry ice
storage temp.: −196°C
biological source:
human foreskin (juvenile epidermis)
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pkg of 500,000 cells
morphology:
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technique(s):
cell culture | mammalian: suitable
shipped in:
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biological source: human pulmonary artery
packaging: pkg of 500,000 cells
morphology: (smooth muscle)
technique(s): cell culture | mammalian: suitable
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biological source:
human pulmonary artery
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pkg of 500,000 cells
morphology:
(smooth muscle)
technique(s):
cell culture | mammalian: suitable
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dry ice
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