Human White Preadipocytes (HWP)

Description

• General description: Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
• Cell Line Origin: Adipose Tissue
• Application: Primary Human White Preadipocytes (HWP) are isolated from adult subcutaneous or visceral adipose tissue from different locations (lot specific source information is available on request) and provided in a cryopreserved format.Adipose tissue is crucial in energy storage and metabolic homeostasis. An increase in adipose tissue results either from an enlargement of mature adipocytes or from the differentiation of adipocyte precursor cells (preadipocytes) into new mature adipocytes. These preadipocytes are already present in adipose tissues. They can proliferate throughout adult life and replace the cells that have differentiated into mature adipocytes. Since this differentiation process is controlled by a variety of growth factors and hormones, preadipocytes are suitable for the investigation of the physiological mechanisms controlling proliferation, differentiation, and function of adipose tissue.
• Quality: Rigid quality control tests are performed for each lot of Human White Preadipocytes. They are tested for cell morphology, adherence rate, and cell viability. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions without antibiotics and antimycotics. Furthermore, each lot of Human White Preadipocytes is extensively tested for its capacity to differentiate into mature adipocytes. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
• Warning: Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
• Subculture Routine: Click here for more information.
• Other Notes: Recommended Plating Density: 5000 cells per cm2Passage After Thawing: P2Tested Markers: Differentiation into adipocytes testedGuaranteed population doublings: >10
• Recommended products: Recommended Primary Cell Culture Media:Link
• Disclaimer: RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.