Description

• General description: Chorionic gonadotropin human (CGA or hCG) is a heterodimer and belongs to the cystine knot growth factor superfamily. It exists as six variants namely hCGn, hCGβ, hCGβn, hCGβcf and hCGα including hCG.[1] CGA is mapped to human chromosome 6q14.3.[2] hCG comprises of α and β chain.[3]
• Application: Chorionic gonadotropin human has been used: to induce oocyte maturation in cultured follicles[4]along with pregnant mare serum gonadotropin (PMSG) to induce superovulation in mice[5] as medium component for fetal testes culture to aid steroidogenic responsiveness[6]
• Biochem/physiol Actions: Elevated levels of hCG is observed in trophoblastic cancer.[7] hCG levels are indicators of pregnancy and β subunit is majorly present in urine samples.[8] hCG isoform detection is useful in the diagnosis and management of trophoblastic related diseases including carcinomas.[3]
• Reconstitution: When reconstituted with water to a concentration of 1,000 IU/mL, the vial will also contain 0.1 M sodium phosphate buffer (pH approx. 7.2) and 100 mg/mL of mannitol.
• Analysis Note: The calculated value is based on the USP method for human Chorionic Gonadotrophin, to meet 5,000 IU per vial, with a tolerance of not less than 80.0% and not more than 125.0% of the potency stated.