H6147
HEPES
Manufacturer: Sigma Aldrich
CAS Number: 7365-45-9
Synonym(S): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 25 G | H6147-25-G | In Stock | ₹ 8,291.70 |
| 100 G | H6147-100-G | In Stock | ₹ 20,823.60 |
| 500 G | H6147-500-G | In Stock | ₹ 62,770.50 |
H6147 - 25 G
In Stock
Quantity
1
Base Price: ₹ 8,291.70
GST (18%): ₹ 1,492.506
Total Price: ₹ 9,784.206
product line
BioXtra
Quality Level
400
Assay
≥99.5% (titration)
form
powder
technique(s)
cell culture | embryo: suitable
pH
5.0-6.5 (25 °C, 238 g/L)
useful pH range
6.8-8.2
pKa (25 °C)
7.57.5
solubility
water: 500 mg/mL, clear, colorless
absorption
≤0.05 at 290 in H2O at 33%
Other Options
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Description
- General description: HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2] It is typically used in cell culture at concentration between 5mM to 30mM.
- Application: HEPES has been used:As a constituent of modified Eagle′s minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells[3]As a component of modified Ham′s F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production[4]in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation[5]
- Other Notes: This product has been qualified for use in cell and mouse embryo culture.
- Physical properties: Useful pH range: 6.8 - 8.2
SAFETY INFORMATION
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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product line: BioXtra
Quality Level: 400
Assay: ≥99.5% (titration)
form: powder
technique(s): cell culture | embryo: suitable
pH: 5.0-6.5 (25 °C, 238 g/L)
useful pH range: 6.8-8.2
pKa (25 °C): 7.57.5
solubility: water: 500 mg/mL, clear, colorless
absorption: ≤0.05 at 290 in H2O at 33%
product line:
BioXtra
Quality Level:
400
Assay:
≥99.5% (titration)
form:
powder
technique(s):
cell culture | embryo: suitable
pH:
5.0-6.5 (25 °C, 238 g/L)
useful pH range:
6.8-8.2
pKa (25 °C):
7.57.5
solubility:
water: 500 mg/mL, clear, colorless
absorption:
≤0.05 at 290 in H2O at 33%
product line: __
Quality Level: 100
Assay: ≥95% (SDS-PAGE)
form: lyophilized powder
technique(s): __
pH: __
useful pH range: __
pKa (25 °C): __
solubility: __
absorption: __
product line:
__
Quality Level:
100
Assay:
≥95% (SDS-PAGE)
form:
lyophilized powder
technique(s):
__
pH:
__
useful pH range:
__
pKa (25 °C):
__
solubility:
__
absorption:
__
product line: __
Quality Level: 200
Assay: __
form: lyophilized powder
technique(s): __
pH: __
useful pH range: __
pKa (25 °C): __
solubility: __
absorption: __
product line:
__
Quality Level:
200
Assay:
__
form:
lyophilized powder
technique(s):
__
pH:
__
useful pH range:
__
pKa (25 °C):
__
solubility:
__
absorption:
__
product line: __
Quality Level: 200
Assay: ≥99%
form: powder or crystals
technique(s): __
pH: 5.0-7 (25 °C, 36.4 g/L)
useful pH range: __
pKa (25 °C): __
solubility: __
absorption: __
product line:
__
Quality Level:
200
Assay:
≥99%
form:
powder or crystals
technique(s):
__
pH:
5.0-7 (25 °C, 36.4 g/L)
useful pH range:
__
pKa (25 °C):
__
solubility:
__
absorption:
__