EmbryoMax® Primary Mouse Embryonic Fibroblasts

Description

• General description: Primary Mouse Embryo Fibroblasts, neo resistant, Strain FVB, are mitotically inactivated by treatment with irradiation and serve as a feeder layer for both mouse and human ES/IPS cells. MEF cells are resistant to neomycin.Plating MEF Feeder CellsProcedure:1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density andappearance. Gelatinized plates may be used for 12–14 days.
• Cell Line Description: Primary Mouse Embryo Fibroblasts
• Application: Research CategoryStem Cell Research
• Packaging: 5-6x106 ea
• Storage and Stability: Vials should be stored in the vapor phase of liquid nitrogen.
• Legal Information: EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany