Gibco™ FreeStyle™ 293 Expression Medium

Description

• FreeStyle™ 293 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F cells in suspension without adaptation
• The medium is complete and ready-to-use, with no supplementation required
• Features of Gibco™ FreeStyle™ 293 Expression Medium include: Complete and ready-to-use format containing GlutaMAX™ supplement Ability to support suspension growth and transfection of 293-F cells Animal origin–free, protein-free and chemically defined formulation Easy use with the FreeStyle™ 293 Expression systems Scalability in spinner flasks and bioreactors Complete and ready-to-use format containing GlutaMAX™ supplement Gibco™ FreeStyle™ 293 Expression Medium is ready to use
• It does not require the addition of serum, glutamine, or surfactants
• Gibco™ FreeStyle™ 293 Expression Medium contains GlutaMAX™ supplement to minimize toxic ammonia build-up and improve cell viability and growth in an easy-to-use format
• Ability to support suspension growth and transfection of 293-F cells Gibco™ 293-F, 293-H, and FreeStyle™ 293-F cells in suspension can be cultured directly into Gibco™ FreeStyle™ 293 Expression Medium without adaptation
• Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco™ FreeStyle™ 293 Expression Medium, usually without any further adaptation
• Cells usually require adaptation from serum-containing formulations
• Cells cultured in Gibco™ FreeStyle™ 293 Expression Medium can be transfected using lipid based transfection reagents, such as 293fectin™ reagent (Cat
• No
• 12347019) and Freestyle™ MAX reagent (16447500)
• Animal origin–free, protein-free and chemically defined formulation Gibco™ FreeStyle™ 293 Expression Medium is animal origin–free, protein-free and chemically defined, allowing for easier purification of your protein of interest
• Gibco™ chemically defined media contain no proteins, hydrolysates, or components of unknown composition
• Easy use as part of the FreeStyle™ 293 Expression System and FreeStyle™ MAX Expression System for protein production The FreeStyle™ 293 Expression System (Cat
• No
• K900001) is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein (see figure)
• The FreeStyle™ 293 Expression System combines Gibco™ FreeStyle™ 293 Expression Medium with 293fectin™ reagent, a cationic-lipid formulation designed to transfect suspension FreeStyle™ 293-F cells at high efficiency
• The FreeStyle™ MAX Expression System (K900010) is a breakthrough technology for rapid and high-yield mammalian protein production
• The FreeStyle™ MAX Expression System combines Gibco™ FreeStyle™ Media, FreeStyle™ MAX Reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293-F cells
• Scalability in spinner flasks and bioreactors Protein production using Gibco™ FreeStyle™ 293 Expression Medium can be scaled up in spinner flasks or bioreactors
• The appropriate spinner or impeller speed and seeding density should be optimized for each system
• Depending on the impeller design and speed, it may be necessary to supplement Gibco™ FreeStyle™ 293 Expression Medium with additional Pluronic™ F-68 to avoid sheer stress in the culture
• Universal bag packaging Gibco™ universal bags offer flexible packaging that allows you to integrate a single-use, disposable large volume media container with a wide variety of automated cell culture instruments, disposable bioreactor systems, and sterile connection processes, minimizing the need for costly customization
• Product use Customers using Gibco™ FreeStyle™ 293 Expression Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF)
• cGMP manufacturing and quality system Gibco™ FreeStyle™ 293 Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York
• The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.