Gibco™ DMEM/F-12, powder, HEPES

Description

• DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells
• Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
• We offer a variety of DMEM/F-12 modifications for a range of cell culture applications
• Find the right formulation using the media selector tool
• This DMEM/F-12 is modified as follows: With: L-glutamine, HEPES, Phenol Red Without: Sodium Bicarbonate The complete formulation is available
• Using DMEM/F-12 DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12
• This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components
• DMEM/F-12 contains no proteins, lipids, or growth factors
• Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO 2 environment to maintain physiological pH
• Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).