Gibco™ Ham's F-12K (Kaighn's) Medium

Description

• Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture
• Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment
• This F-12K (Kaighn's) Medium is manufactured as follows: With: L-glutamine, Phenol Red Without: HEPES The complete formulation is available
• Using Ham's F-12K Ham's F-12K (Kaighn's) Medium contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate
• These additions allow the medium to be supplemented with very low levels of serum or defined components, for some cell types
• Ham's F-12K (Kaighn's) Medium contains no proteins or growth factors, and is therefore often supplemented with growth factors and Fetal Bovine Serum (FBS)
• The FBS concentration must be optimized for each cell line
• Ham's F-12K (Kaighn's) Medium uses a sodium bicarbonate buffer system (2.5 g/L), and therefore requires a 5–10% CO 2 environment to maintain physiological pH
• Order Info Shipping Condition: Room Temperature