Gibco™ Medium 199 (2X)

Description

• Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts
• It has broad species applicability, particularly for cultivation of non-transformed cells
• Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues
• We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications
• Find the right formulation using the media selector tool
• This Medium 199 is modified as follows: With: L-glutamine Without: HEPES, Phenol Red The complete formulation is available
• Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins
• Medium 199 is available with Earle's salts for use in a CO 2 incubator, or with Hanks' salts for use without CO 2
• This Medium 199 is made with Earle's salts
• Medium 199 contains no proteins, lipids, or growth factors
• Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO 2 environment to maintain physiological pH.