Gibco™ Ham's F-12 Nutrient Mix, powder

Description

• Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells
• F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells
• We offer a variety of Gibco™ F-12 modifications for a range of cell culture applications
• Find the right formulation using the media selector tool
• This Ham's F-12 is modified as follows: With: L-glutamine, Phenol Red Without: Sodium Bicarbonate The complete formulation is available
• Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine
• Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations
• Gibco™ F-12 contains no proteins or growth factors
• Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO 2 environment to maintain physiological pH
• Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details)
• Order Info Shipping Condition: Room Temperature Compliance Gibco F-12 is manufactured at a cGMP compliant facility located in Grand Island, New York
• The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.