Gibco™ RPMI 1640 Medium (Dutch modification)

Description

• Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer
• RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas
• We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications
• Find the right formulation using the media selector tool
• This RPMI is modified as follows: With: Phenol Red, HEPES, Low Sodium Bicarbonate Without: L-glutamine The complete formulation is available
• Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins
• RPMI 1640 contains biotin, vitamin B 12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
• In addition, the vitamins inositol and choline are present in very high concentrations
• This RPMI formulation has the Dutch modification (addition of HEPES, with sodium bicarbonate lowered to 1 g/L)
• Product Intended Use For Research Use Only
• Not for use in diagnostic procedures
• RPMI 1640 contains no proteins, lipids, or growth factors
• Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• RPMI 1640 uses a sodium bicarbonate buffer system (1.0 g/L) and therefore requires a 5-7% CO 2 environment to maintain physiological pH
• Order Info Shipping Condition: Room Temperature Compliance Gibco RPMI 1640 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK
• The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.