Gibco™ Essential 8™ Medium

Description

• Essential 8™ Medium is a xeno-free and feeder-free medium specially formulated for the growth and expansion of human pluripotent stem cells (PSCs)
• Originally developed by Guokai Chen et al
• in the laboratory of James Thomson (published as 'E8') and validated by Cellular Dynamics International, Essential 8™ Medium has been extensively tested and proved to maintain pluripotency in multiple iPSC lines
• In addition, Essential 8™ Medium has been used to scale up production of iPSCs and shown to support iPSC growth for >50 passages without any signs of karyotypic abnormalities, along with maintaining the ability of iPSCs to differentiate into all three germ line lineages
• Features of Essential 8™ Medium include: Consistent—reduced variability compared to existing feeder-free culture media Robust—reliable and robust cultures with a xeno-free, cGMP, 8-component medium Cost effective—economical and scalable PSC culture compared to other feeder-free media Reduced Variability Essential 8™ Medium is xeno-free and contains only the 8 essential components needed for stem cell culture
• Unlike other media that contain over 20 highly variable ingredients, Essential 8™ Medium is produced under cGMP and has an optimized formulation and growth factor levels to help ensure maximum cell health, pluripotency, and growth, with minimal variability
• Reliable and Robust Cultures Essential 8™ Medium has been show to support pluripotent stem cell growth and provide cultures with superior morphology and growth kinetics compared to other feeder systems
• Cost Effective Essential 8™ Medium is provided in a convenient two-component kit (500 ml basal medium & 10 ml supplement), and when used with vitronectin (VTN-N) , provides a cost effective, defined system for feeder-free culture of human pluripotent stem cells (PSCs)
• Essential 8™ Medium is commercialized in partnership with Cellular Dynamics International.