Gibco™ SILAC RPMI 1640 Flex Media, no glucose, no phenol red

Description

• SILAC RPMI 1640 Medium is RPMI 1640 basal cell culture medium without L-arginine, L-glutamine, and L-lysine for use during SILAC protein labeling with stable isotope-labeled lysine and/or arginine
• RPMI 1640 Medium is widely used for supporting the growth of many different mammalian cells
• Cells successfully cultured in RPMI-1640 include HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas
• We offer a variety of Gibco™ RPMI 1640 modifications for a range of applications
• Find the right modification using the media selector tool
• RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins
• RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
• In addition, the vitamins inositol and choline are present in very high concentrations
• RPMI 1640 Medium contains no proteins, lipids, or growth factors
• Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• SILAC protein labeling experiments should be conducted using Gibco™ Dialyzed FBS
• RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5-10% CO 2 environment to maintain physiological pH
• cGMP Manufacturing and Quality System SILAC RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York
• The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.