Gibco™ BenchStable™ RPMI 1640

Description

• Gibco RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer
• RPMI 1640 medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas
• We offer a variety of RPMI 1640 modifications for a range of cell culture applications
• Find the right formulation using the media selector tool
• BenchStable RPMI 1640 Medium has been engineered for storage at room temperature while supporting cellular proliferation and maximum cell densities comparable to conventional RPMI 1640 Medium when supplemented with ≥10% fetal bovine serum (FBS)
• This RPMI 1640 Medium is modified as follows: • Optimized for room temperature stability • With GlutaMAX, Phenol Red • Without HEPES Using BenchStable RPMI 1640 Medium RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins
• RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
• In addition, the vitamins inositol and choline are present in very high concentrations
• RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L) and so requires a 5–10% CO2 environment to maintain physiological pH
• RPMI 1640 Medium with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format
• BenchStable RPMI 1640 Medium contains no proteins, lipids, or growth factors and so requires supplementation with ≥10% fetal bovine serum (FBS).