Gibco™ CultureCEPT™ Supplement (1000X)

Description

• Gibco CultureCEPT Supplement reduces cellular stress and improves viability of stem cells, differentiated cells, and primary cells during a wide variety of handling and processing steps where cell damage and death can limit the success of a cell culture workflow
• It is a unique formulation that provides superior cell protection compared to traditional ROCK inhibitors, such as Y-27632, and other commercially available cell culture reagents
• The formulation is based on advancements from the Stem Cell Translation Laboratory at the NIH's National Center for Advancing Translational Sciences (NCATS), where a screen of over 15,000 compounds identified a combination of small molecules (referred to as CEPT) that work together to provide unmatched cytoprotection in passaging pluripotent stem cells, embryoid body creation, organoid formation, single-cell cloning, gene-editing, differentiation, and recovery of cryopreserved cells including PSCs, cardiomyocytes, neurons, astrocytes, and hepatocytes
• CultureCEPT Supplement contains the CEPT cocktail molecules, chroman 1, emricasan, polyamines, and trans-ISRIB, to provide improved cell viability
• Achieve unparalleled cell survival in numerous applications CultureCEPT Supplement will benefit scientists looking for: • Improved viability of PSCs, differentiated cells, or primary cells following recovery from cryopreservation • Improved viability during the passaging of stem cells • Improved viability during electroporation or transfection • Improved clonal efficiency during gene-editing and clonal selection with PSCs • Improved embryoid body and organoid formation CultureCEPT Supplement harnesses the power of CEPT • Innovative small molecules comprising the CEPT cocktail have been extensively tested by the NIH's NCATS (Chen et al., 2021) • Growing number of publications highlights utility and exceptional performance of CEPT small molecules Compatible with trusted Gibco media systems • Evaluated in StemScale PSC Suspension Medium, StemFlex Medium, Essential 8 Medium, Essential 8 Flex Medium, Neurobasal Medium, and Neurobasal Plus Medium • For over 60 years Gibco reagents have enabled successful cell culture Ready-to-use • Fully formulated 1000X • Simply add directly to media • No weighing, no resuspending, no displacing media, no hassle Citations and references Chen Y, et al
• A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells
• Nat Methods
• 2021 May;18(5):528-541
• Tristan CA, et al
• Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail
• Nat Protoc
• 2023 Jan;18(1):58-80
• Ryu S, et al
• Stress-free cell aggregation by using the CEPT cocktail enhances embryoid body and organoid fitness
• Biofabrication
• 2023 Dec;16(1):10.1088/1758-5090/ad0d13
• Deng T, et al
• Scalable generation of sensory neurons from human pluripotent stem cells
• Stem Cell Reports
• 2023 Apr 11;18(4):1030-1047
• Jovanovic VM, et al
• A defined roadmap of radial glia and astrocyte differentiation from human pluripotent stem cells
• Stem Cell Reports
• 2023 Aug;18(8):1701-1720
• McIntire E, et al
• Guided Differentiation of Pluripotent Stem Cells for Cardiac Cell Diversity
• Preprint
• bioRxiv
• 2024 Feb;2023.07.21.550072
• Jin M, et al
• Generation of iPSC-based human-mouse microglial brain chimeras to study senescence of human microglia
• STAR Protoc
• 2022 Dec;3(4):101847
• Liu L, et al
• Modeling post-implantation stages of human development into early organogenesis with stem-cell-derived peri-gastruloids
• Cell
• 2023 Aug;186(18):3776-3792.e16
• Kang SY, et al
• A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis
• Adv Healthc Mater
• Published online August 24, 2023
• Deng C, et al
• Massively parallel characterization of psychiatric disorder-associated and cell-type-specific regulatory elements in the developing human cortex
• Preprint
• bioRxiv
• 2023 Feb;2023.02.15.528663
• Tristan CA, et al
• Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells
• Stem Cell Reports
• 2021 Dec 14;16(12):3076-3092
• Ryu S, et al
• Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules
• Methods Mol Biol
• 2022;2454:811-827
• Held A, Adler M, Marques C, et al
• iPSC motor neurons, but not other derived cell types, capture gene expression changes in postmortem sporadic ALS motor neurons
• Cell Rep
• 2023 Sep;42(9):113046.