Description

• General description: Pre-activated reagent for the introduction of biotin by solid phase synthesis. More soluble and more reactive than 851023 [1]. This reagents has recently been found to be effective in SPOT synthesis of biotinylated peptides [2]. Associated Protocols and Technical ArticlesBiotinylation Reagents for Peptide SynthesisAutomate your Biotin tagging with Synple Automated Synthesis Platform (SYNPLE-SC002)Literature references[1] B. Baumeister, et al. Int. J. Pept. Res. Ther., 11, 139. [2] D. Winkler & P. McGeer (2008) Proteomics, 8, 961.
• Application: Mechanistic studies of a cell-permeant peptide designed to enhance myosin light chain phosphorylation: This study discusses the synthesis and applications of cell-permeant peptides involving biotin-ONP for enhanced cellular processes (Almansour, 2017). Structure-function analysis of tight junction-directed permeation enhancer PIP250: Provides insights into the utility of biotin-ONP in analyzing the structure and function of peptides used to enhance cellular permeation (Taverner et al., 2023).
• Linkage: Replaces: 01-63-0116
• Analysis Note: Color (visual): white to slight yellow to beigeAppearance of substance (visual): powderIdentity (IR): passes testOptical rotation α 25/D (c=1 in dioxan): +34.5 - +39.5 °Purity (TLC(0811)): ≥ 98 %Assay (HPLC, area%): ≥ 97.0 % (a/a)To see the solvent systems used for TLC of Novabiochem® products please click here.
• Legal Information: Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany